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NegativeStain

Unique features

  • This protocol is used to screen and acquire data on Lacey carbon, Negative stain grid or other non-holey grids.
  • Instead of attempting to find holes, a regular lattice of targets is layed on top of the squares and are used as targets.

    The lattice spacing is 5 um.

  • There is no hole recentering at the medium magnification either.